With that said, the challenge that remains with the application of DLS to protein therapeutic formulations is centered around data interpretation. The popularity of DLS within the biopharmaceutical industry is a consequence of its wide working size and extended sample concentration ranges, as well as its low volume requirements. With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions.Dynamic light scattering (DLS) is an analytical technique used to measure the particle size distribution of protein formulations across the oligomer and sub-micron size ranges of approximately 1 nm to 1 µm. Wavelength(s) With standard SEC/GPC or Eclipse™ FFF system: determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration.R h(hydrodynamic radius): 0.5 nm to 300 nm (with optional DLS module, upper limit configuration-dependent) Measuring Range Molar mass: 200 Da to 1000 MDa.Optional: 785 nm for fluorescence suppression Light Source 658 nm laser, programmable 10% - 100% of maximum power.Detector(s) High-gain, high-dynamic-range photodiodes at 18 angles for MALS optional avalanche photodiode that may be placed at any of four angles for DLS.įluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength.Type Multi-angle Light Scattering (MALS) and optional dynamic light scattering (DLS), online or in a microcuvette.Item DAWN® Multi-angle Light Scattering (MALS) Instrument.
#DYNAMIC LIGHT SCATTERING SIZE RANGE SOFTWARE#
The product is a accompanied by ASTRA®, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. Includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics, even with the host computer turned off. It can also be used with the Calypso® system to characterize biomolecular interactions. May be used with ion-exchange chromatography or reverse-phase chromatography to detetrmine the molecular weight of eluting peaks. The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS) / size exclusion chromatography (SEC-MALS) or an Eclipse™ field-flow fractionation instrument (FFF-MALS) to determine distributions of mass, size, conformation, composition and particle concentration, independent of column calibration by reference standards. It offers the highest sensitivity, the widest range of molecular weight, size and concentration, and the largest selection of configurations and optional modules for enhanced capabilities of any MALS instrument. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, and conjugation e.g. The world's most advanced light scattering instrument for absolute characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles.
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